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楼主  发表于: 2009-10-18   

医学SCI 论文经典句子

18 A*tKF&U5  
Jurkat cells cultured in calcium-free RPMI-1640 medium (Gibco BRL; number !q"CV  
22300-107) containing calcium-free 10% FBS were triggered by anti-Fas IgM. The ~svO*o Wa  
treated cells were harvested at the indicated time points and incubated with Fluo-3-AM at +{53a_q  
a final concentration of 1 micromolar for 30 min at 37°C (Scoltock et al., 2000). The 4F MAz^  
labeled cells (50,000 cells per treatment) were then analyzed by exciting the cells at 488 fYrGpW( `  
nm and examining the fluorescence emission of Fluo 3 at 530 nm with a FACS Scan, [t0rfl{.  
(Becton Dickinson). A one micromolar concentration of LPA was used as a positive eX l=i-'  
control for Ca2+ induction. The data thus obtained was analyzed with the software Win !~-6wN"k  
MDI 2.8 and represented as contour plots.The effect of chelating intracellular calcium on 9x`4 RE  
translocation of annexin I was studied by culturing Jurkat cells in the presence of 10 )yxT+g2!  
micromolar BAPTA-AM, with or without the addition of anti-Fas IgM. Cells were 5 ZfP  
harvested and fractionated as detailed above, and the S-100 fractions were assessed by v@EQ^C2.&  
immunoblotting for presence or absence of annexin I. dX )W0  
Mouse bone marrow transduction and transplantation. ,7d|O}B  
Retrovirus-mediated transduction of mouse bone marrow cells was done by published g.Hio.fVd  
methods49. Prestimulated low-density bone marrow cells were infected with high-titer pvXcLR)L+3  
retrovirus supernatant on fibronectin-coated plates. Retrovirus supernatant was generated Nf?\AK!  
in the phoenix-gp cells with a mouse stem cell virus–based retroviral vector coexpressing >xd<YwXZ  
EGFP and HA–RhoH as described50. EGFP+ sorted cells were transplanted by {0's~U+@  
intravenous injection into the sublethally irradiated (300 rads with a 137Cs irradiator) YL5>V$i  
Rag2-/- recipient mice. At 9 weeks after transplantation, thymus, peripheral blood, bone ^ @.G,u  
marrow, spleen and lymph nodes from each recipient mouse were collected for analysis D[>W{g $  
of EGFP+ chimerism and hematopoietic lineage by flow cytometry. Expression of d\Jji 6W  
HA–RhoH and HA–RhoHF73F83 in EGFP+ sorted thymocytes of recipient mice was 3ps,uozj  
confirmed by immunoblot analysis. \f"?Tv-C '  
Determination of renal morphology  Tb#  
Kidney slices were postfixed in buffered 2% OsO4, dehydrated, and embedded in an a c6*v49  
Araldite-EM bed 812 mixture. Large sections were cut perpendicular to the renal capsule, ="B n=>  
containing cortex, and medulla. Thin (1 m) sections were analyzed in a blinded manner oBw}hH,hp  
for morphologic alterations, as previously detailed |s`Kd-'|q  
Patient population v*L '{3f  
Patients included in the study met the following criteria: (1) biopsy-proven IMN; (2) 'rx?hL3VW  
creatinine clearance 30 ml per min per 1.73 m2; and (3) persistent proteinuria >5 g per W uf/LKj  
24 h despite treatment with an HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) 9cOx@c+/  
reductase inhibitor, an ACEi, and/or ARB at maximal tolerated dose for at least 4 months. #HDe sen  
The Mayo Institutional Review Board and the Research Ethical Board, University Health {]M>Y%j48  
Network, University of Toronto approved the study protocol. All patients gave written bs:QG1*.  
informed consent. Patients who had been on treatment with prednisone, cyclosporine, or 08io<c,L  
19 m >hovikY*  
mycophenolic mofetil within the last 4 months or alkylating agents within the last 6 gA:[3J,[;  
months were not included in the study. Patients with active infection, diabetes, or a SW=aHM   
secondary cause of MN (for example, hepatitis B, systemic lupus erythematosus (SLE), q]+)c2M  
medications, malignancies) were also excluded. TnxU/)  
Treatment V7n >,k5  
At enrollment, a low-sodium (<4 g day-1) and low-protein (0.8 g per kg per day of @? vLAsp\  
high-quality protein) diet was recommended and patients were encouraged to maintain `~|DoSi^d  
the same diet throughout the duration of the study. All patients received a similar Kv1vx*>  
conservative treatment regimen that included loop diuretics to control edema, an X -v~o/r7  
HMG-CoA reductase inhibitor, and an ACEi combined with an ARB if tolerated. Gzj3Ka  
-Blockers and non-dihydropyridine calcium channel blockers, in that order, were added 7Qt2gf  
when required to control systolic blood pressures to <135 mm Hg in >75% of the tgK x4  
readings. Patients who after a minimum of 4 months of conservative therapy and G+fd.~aGE  
maximized Ang II blockade had proteinuria >5 g per 24 h received two i.v. infusions of  .?70=8{  
rituximab at a dose of 1000 mg on days 1 and 15. To minimize infusion reactions, 4 ;^g MI9  
patients were premedicated with acetaminophen (1000 mg) and diphenhydramine XEa~)i{O  
hydrochloride (50 mg) orally. In addition, methylprednisolone (100 mg, i.v.) was given ~DK F%}E  
prior to the first rituximab infusion. B-cell depletion was defined as CD19+ count { _-wG3f|  
<5 cells per l at any time and B-cell recovery was defined as CD19+ cell count zU=YNrn  
>15 cells per l. Patients treated with rituximab, who at month 6 had proteinuria >3 g per a (P^e)<  
24 h and in whom CD19+ B-cell counts had increased to >15 cells per l, received a F ZM2   
second course of rituximab treatment following the same protocol described above. jGJ.Pvc>i  
Follow-up N\c &PS  
In all patients, clinical and laboratory parameters including complete blood counts, =rtS#u Y  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum p1Y +  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry Nvi Fq  
and at months 1, 3, 6, 9, and 12. Creatinine clearance and protein and creatinine excretion {qJHL;mP:8  
in the urine were assessed by performing two consecutive 24-h urine collections at each \I'f3  
time point. Data were considered accurate when urinary creatinine excretion was pk;ffq@  
consistent with a complete 24 h collection. The mean of the two measurements was sM #!Xl;  
considered for the analysis. The presence of HACAs was evaluated at baseline and at t |go5DXz4  
months 3, 6, 9, and 12. JZW gr&O<  
Method / Approach / Study/ Technique |\i:LG1  
A discussion is presented of a problem-solving system fG X1y  
To improve the efficiency of the method, the following approach may be applied. q"{Up  
In order to an investigation was made to find the causes of the 7( yXsVq  
Although large collections of rules and equations have been complied, none are generally omXBnzT  
accepted 83{x"G3>  
20 +io;K]C  
This approach will be explained and discussed thoroughly in the body of the report. L4\SB O  
This can be accomplished by }2-[Ki yv  
This algorithm to compute the total cost can be described step by step as follows:  1m&!l6Jk  
The above preliminary analysis has provided important information Nm H}"ndv+  
Various methods have been proposed for selecting an optimum... `ENlV9  
These concepts have been applied to o|rGy 5  
On the basis of the concept mentioned above, S(g<<Te  
This can be achieved by %f_)<NP9=  
In addition, tissues were stained for infiltrating lymphocytes (CD20 and CD3), and the X";QA":  
amount of interstitial fibrosis was quantified by histomorphometry. Formalin-fixed, hW0,5>[7%  
paraffin-embedded sections were cut onto coated glass slides. Following heat-induced b9W<1eqF  
antigen retrieval, sections were incubated at 20°C overnight with either anti-CD20 5~`|)~FA  
primary antibody or anti-CD3 primary antibody, both at 1:1000 dilution (Dako, Canada t7U,AQ=;P5  
Inc, Mississauga, Ontario, Canada). After rinsing all sections, pretreatment with 3% 9 NGeh*`  
hydrogen peroxide was performed to prevent endogenous peroxidase activation. Sections SX Hru Z  
were incubated with a secondary rabbit anti-mouse antibody linked with avidin–biotin L]/\C{}k  
complex. Sections were counterstained with hematoxylin and examined by light ; JPb Bwm  
microscopy. !T#8N7J>  
The HACA assay is a proprietary bridging enzyme-linked immunosorbent assay `>`K7-H  
performed at Genentech Inc. that measures the antibody response to rituximab in human v$.JmL0^J  
serum samples. j{7ilo(i  
In all patients, clinical and laboratory parameters including complete blood counts, 6*(h9!_T1  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum W[1f]w3  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry Ads<-.R  
and at months 1, 3, 6, 9, and 12. <u x*r#a!d  
This fact suggests that a new concept xnhDW7m  
This was accomplished by taking ... P$(iB.&  
The preparatory stage is very time consuming process. ! 6p)t[s  
Test are performed for validity, completeness, and compatibility cvs" WX3  
There is little hope of achieving successful ... X#Dhk6  
There has been an increasing awareness of the potential of using most ..so far made have o7 @4=m}  
not taken this approach, with the exception of 1EAVMJ  
Only a few studies can be found. ;@7 #w  
It is a very tedious process to go through KWigMh\r  
It is only when .. has been completed that .. may be effected hPqapz]HcP  
The entire interpretation process is conducted in one's head. 1n"+~N^\  
These approaches are sometimes very tedious. vRb(eg  
Several techniques can be used ,QHx*~9  
A polynomial parametric model can be written as [the following]/[follows]: &J&w4"0N'  
A xx model is constructed/formulated using xx. QhE(" }1  
A xx model represents an xx by its xx. 1O3<%T#LOZ  
A process decision model captures the logic essential to l~6SR  
From the equation above, xx is equal to the summation of xx times the ... c;~Llj P  
21 RH'F<!p  
The validity of a xx model can be checked using Euler's formula. #u=O 5%.  
Given a model, one can mathematically determine whether ... or ... | C2.Zay  
Equations for xx need to be derived and implemented in the system. 7jg(j~tQ  
A number of heuristic rules have been developed for ah6F^Kpl{  
Optimum .. techniques can be made more reliable by ... so that K[|P6J   
An algorithm based on the characteristic ... is used to determine O"-PNF,J  
Euler's formula states the following: C^42=?  
The completed model should agree with the formula. Jp*AIj  
For manufacturing purposes, a detailed and precise model of the object is necessary ?>\]%$5o  
Engineering design models are very well defined; therefore, 53&xTcv}x  
To keep the domain narrow enough to be implementable, yet wide enough to be useful. Tx?@* Q  
Point of View ]jY)M<:J4  
from an implementation standpoint, "yq;{AGOGl  
From the point of view of this application, hE#8_34%s  
From this point of view, Zadeh suggested an inference rule named xxx (CRI for short). S"A_TH  
Information is the meaningful interpretation and correlation of some aggregation of data -Jrc'e 4K  
in order to allow one to make decisions. &+{xR79+&  
From a practical point of view, the computational aspects of an FLC require a 9C~GL,uKs  
simplification of the fuzzy control algorithm. nMoF;AdKm  
The use of a hammer to insert screws, although partly effective, tends to distort, destroy, td$6:)   
and generally defeat the purpose of using a screw [Kusiak AI Implications for CIM s ;Nu2aOp7  
p.129] (9_e >2_  
Statistical analysis g\nL n#  
Data were analyzed by one-way analysis of variance comparing the three conditions n?fC_dy  
(sham operation, ischemic AKI, and bilateral nephrectomy) at each time point. If rtOW-cz  
significant F-statistic from analysis of variance existed, this test was followed by Dunnett _r)nbQm&  
post hoc multiple comparison procedure with sham operation as the control group. For all yXl.Gq>]{  
other comparisons, Student's t-test was used. A P-value of <0.05 was considered as /38Pp%  
statistically significant. (8F?yBu  
Values are expressed as means s.e.m. and significance was evaluated by Mann–Whitney +mPB?5  
U test using GraphPad Prism, version 4.0 software (GraphPad Software Inc., San Diego, ,\RCgc  
CA, USA). IN*Z__l8j`  
All values are expressed as means s.d. Statistical significance (defined as P<0.05) was ~%q e,  
evaluated using analysis of variance and Bonferroni t-tests, and the two-tailed Pearson's ht!:e>z&4  
test, where appropriate. j+v)I =  
Data are expressed as mean s.d., median and interquartile range, or frequencies, as >CA1Ub&ls  
appropriate. Variables who deviated from the normal distribution (positively skewed) +eat,3Ji  
were log-transformed (log10) before the correlation study. |0Kt@ AJY  
Data are represented as means s.e.m. Student's t-test and multiple comparisons with t-test [)KfRk?};2  
post hoc analysis of variance were used as indicated below, for the comparison of I!%@|[ Ow  
22 ]vG)lY.=  
morphological, immunohistological and functional parameters. Statistical significance (9h{6rc=I  
was set at P<0.05. 'c]&{-w<i  
The primary efficacy parameter was defined as change in urinary protein excretion from V!4E(sX  
baseline (week 0) to 12 months after treatment. The 12-month changes were tested DenCD9 f  
against zero using the paired t-test. Secondary end points included 6-month changes in 83|/sWrvh  
protein; the number with PR or CR at 6 or 12 months; and changes in glomerular =!2   
filtration rate (GFR), serum albumin, and lipid profiles. Study sample size was based on Ei!z? sxzx  
the desire to have 80% power to detect a drop in urinary protein of at least 2.0 g day-1. +z_0?x  
Assuming a two-sided hypothesis test performed at a significance level of 0.05 and an s.d. TlJF{ <E  
of urinary protein change of 2.5 g, it was determined that 15 patients were required.2 _7VU ,  
Definition of remission status is according to the criteria established by Cattran et al.30 MiI7s ;  
CR was defined as proteinuria <0.3 g per 24 h, PR as proteinuria 3 g per 24 h, and a a7R7Ks|q  
>50% reduction in peak proteinuria and non-response as <50% reduction in peak /fEXAk  
proteinuria. Any patient reaching a CR or PR was considered a treatment success GWsF W[T?~  
The statistical significance of differences for the mean values of cytokine concentration 4D5)<3N=d'  
and T cell proliferation was determined with Student's t-test. Differences with a P value z%z$'m  
of less than 0.05 were considered significant. ?uLqB@!2  
Statistical significance was determined by Student's t-test. Data with a P value of less hi%>&i*  
than 0.05 were considered significant. ;F- kE4w  
In vitro and in vivo experiments were analyzed by the two-tailed Student's t-test, with P c'C2V9t  
values less than 0.05 considered statistically significant. CS  
The significance of differences among groups was determined by Student's t-test and 5;+Bl@zGu  
one-way analysis of variance (SigmaStat software; Jandel). bk 2vce&  
Comparisons /T0|<r!c  
As well, the pros and cons of these representations from a process planning point of view 9 (Z) c  
will be discussed. I[/u5V_b'  
The method of using xx to implement xx described by Zadeh (1973) appeared more asb") NfIm  
suitable ay2.C BF  
As discussed [in the previous section]/[preciously], 6K6ihR!d  
Relation }5|uA/B  
We can not invert F' directly because it defines a many-to-one mapping. JL1Whf  
The relationships appear very complicate %q@@0qenv  
Lifting tasks involve complex and imprecise relationship between the task variables and z\a#"2(G.  
the human operator's characteristics. 2KU [Yd  
These methods are based on the relationship between ... and ... u+N[Cgh  
The fundamental concept of a fuzzy rating language is that we can establish a relationship -Rmz`yOq}  
among terms such as high, medium, and low, and then modify these relationships. +g*Ko@]m>  
This article will thus mention the latter as well as the former. -LF^u;s8&S  
The former two bear a close relation to a fuzzy Cartesian product. 9d v+u6)  
Importance Xln'~5~)  
) Limt<S  
23 MD&Ebq5V  
The emphasis is on an implementation of a general approach to rule based decision E[ ,Ur`>:  
making. s i C/k*  
Consideration / Attention cxVnlgq1  
Careful evaluation is necessary to ensure 0q81H./3  
Such a formulation does not change further considerations. /Ue~W, |  
Considerable attention has been paid to vU::dr  
Attention should be paid to an important finding of this investigation. tONxV`  
Caution should be exercised in this process to avoid ... \ qKh9  
Primary consideration is given to ... components, though others can be accommodated :9 .ik  
After ... has been defined by ..., a carefully analysis is carried out/performed to determine )jvYJ9s  
A number of factors such as ...need to be taken into consideration before making the M1^pf<!s  
appropriate decision. nF]lSg&]X  
It should be noted that Bf.@B0\  
It is important to point out that ... h",kA(+P  
These considerations have heightened interest in the possibility of providing ... R0vWj9nPh  
We should stress the fundamental importance of the xx UH2fP G  
Results. 7xqTTN6h  
Advantages / Disadvantage [WW3'= e^  
One of the major advantages of this new measure of xx is that it can be applied to the @uXF(KDX  
experimental study of #!qa#.Yi  
One advantage of using a .. is the ease of preparing it. @p~f*b4H?  
It has a very fast decision making process %v5IR  
All the algorithms involve mostly logical operations. jInI%  
It can be easily and without additional cost implemented in a microprocessor;based `saDeur#X  
environment. nB>C3e  
It can reduce the waste of designing from scratch. = wEU+R_#o  
The advantages of using a xx to represent xx are the following: Z7)la |  
However, xx is not without its shortcomings. j S~W cu  
In most cases, the xxx shows an improvement over the existing xxx. P3Ocfpf Bp  
Compared to the existing xx, the impacts of the xx are generally reduced by 5% to 9%. 4E:kDl*@  
The "best case" results shows a savings of 6% to 9%. I8M^]+c  
Most of the existing works based on xx approach can only recognize a xx . =r=?N\7I  
Most of the above methods are computational expansive and limited to xx. rs`"Kz`(  
Some other advantages of xx are the following: PLoD^3uG)  
The problem is the limitation of this method to a limited domain of parts. 'wA4}f  
It proved limited in application because it demanded precision in system modeling that df9 jT?l  
was impossible in practice. | 6/ # H*  
There are advantages to be gained in the structuring of costs and benefits, the use of xx, $<Dcb JW  
The disadvantages of this method are also disadvantages of conventional xx approaches. ^&8FwV]  
This combines the best features of both techniques 1>)q 5D  
24 .0=VQU  
Hopefully, this tool can be as the reference framework of for developing a xx platform, V;@kWE>3  
and helping the administration, marketing, and knowledge management activities in dAba'|Y  
virtual communities. p#A{.6Pa:  
Results nA1059B  
An improvement on the result shown above can be made by based on the data provided 4s_|6{ANS  
Recent work has identified 0cF +4,5  
Time-dependent changes of +5<]s+4T  
Cyclosporine-induced cell death is triggered by a non-classical phosphoinositide 3-kinase `2I<V7SF$  
and does not require ERK activation. =sFLzAu8  
Much of the current work on =wcqCW,]  
Discussion of these theories is beyond the scope of this review p3`odmbN  
Based on the information contained in this q{RH/. l  
The result can be categorized into nine classes Geyd VT-  
The results are illustrated by an example XLq%nVBM8\  
The experimental results for each xx time are reported in Table 2. HAca'!p  
From the results obtained so far, it seem that 0x1#^dII  
Because of the inaccuracy of the ..., a conclusion cannot be drawn as Ex`!C]sQ  
Although much effort has been made to., this reality is far from completion. #)7THx/=  
The results indicate that the total benefits are higher than the total costs. -1z<,IN+  
Their results may then serve as guidelines for lower level models, less fuzzy and more }<7S% ?TY  
detailed. I}hY @  
Conclusion *= ;M',nx  
From the discussion, one may conclude that ... <n|ayxA)  
The first indication that fBw"<J{  
In summary, we have shown that 6aMG!_jC  
These results suggested that !M*$p Qi}  
Our findings have shown that X8uAwHa6F  
Our observations have provided 7}%Z>  
This study reveals following five main findings: zMI_8lNz  
To our knowledge, this is the first immunohistochemical report of both PIM and HIFs in 7,BULs\g  
the diabetic kidney. F15Yn  
As mentioned above, <>?7veN92  
Moreover, we demonstrate that, Z^_-LX:%  
A number of studies have elaborated a body of knowledge about q1_iV.G<  
There are findings to indicate that Mq,2S  
On a descriptive level, there is agreement that for c80!Ub@  
One issue that became particularly evident from our study is }Y`D^z~  
Our data are in agreement with the findings and models presented by previous K?$|Y-_D^M  
publications, in particular those of Jones et al 2, R5mL$  
According to our observations, viXt]0  
…was not evident from our data hztxs vw  
25 AR?1_]"=  
It could therefore be concluded that i8Xz'Sw07  
our data illustrate that |SSe n#PYp  
All these different aspects, as listed above, lead to ic=tVs  
In fact, it has already been speculated that }_|qDMk+  
It should be furthermore mentioned in this context that ,We'A R3X  
To identify and verify the essential factors triggering developmental renin expression, it P,W(9&KM  
is of interest now to study the development of intrarenal renin expression in mice with H7?Sd(U  
defined gene mutations, using the results of this study as a reference system. pz0Q@n/X  
In conclusion, using several approaches, we demonstrated that N;mJHr3[F  
But we have not provided formal proof yet that _2~+ %{/m,  
In summary, our results suggest that 5[0W+W  
In conclusion, we identify W'! I+nh  
A better understanding of such regulatory systems may yield novel therapeutic hq*"S -N  
approaches to glomerular diseases. 5 iZx -M  
Preliminary results of short-term studies (2 weeks) indicate that mPJ@hr%3  
However, we believe that 32z2c:G  
However, despite our extensive measurements of these parameters, we found no 1<D^+FC4b,  
relationship between response and initial proteinuria, time to B-cell recovery, the )Nt'Z*K*  
development of antibodies to rituximab, B-cell count in the kidney tissue, nor the degree 9+"ISXS  
of tubular interstitial damage present. A clear conclusion for efficacy, however, cannot be +HkEbR'G0  
obtained from an uncontrolled study, and definitive claims of efficacy should be reserved r,Tq";N'  
for rigorous, prospective, controlled, and randomized trials with the use of rituximab and OfPv'rW{x  
that will also look for factors that may determine its efficacy in IMN. 'b:UafV  
It has become increasingly clear that (Qd@Q,@(s  
Form the above discussion, the conclusion can be reached that jZR2Nx}16  
The conclusions drawn are also valid [PH56f  
In conclusion to this, it becomes obvious that the problem of xx lies not only in... %@^9(xTE  
We have attempted to introduce some concepts associated with a theory of oD 3Q{ e  
Considerable more work, hopefully, will be done in this area Z+agS8e(  
Employing the compositional rule of inference, the assessment of the xx compatibility in Cig! 3  
achieving prescribed xx projectiles in any level of the hierarchy is made possible. 4$ ^rzAi5  
This paper has presented a theoretical and experimental study of the xx process and xx i?n#ge  
concept. 8I<LZ{a10  
The experimental research results will hopefully serve as useful feedback information for JlE b  
improvements for xx work. N**" u"CX  
The scope of this contribution was to introduce a xx method. Bxk2P<d  
This has a tremendous impact on our understanding of Dpkc9~z  
Significant progress has been made in advancing RNAi therapeutics in a remarkably Xp~O?2:3l  
short period of time li n  
To date, little is known about… cdTG ] n  
Irrespective of the mechanisms and the molecules –which are still largely unknown – ~' q&rvk`  
involved in MSC functions, current data suggest that O6]X\Cwj%  
To better understand ?<~P)aVVj  
26 v2}>/b)  
Future Research (常用于conclustion 的结尾) b ix}#M  
Thus, first extension of the approach could be, Rip[  
Further studies are needed to determine the importance of Y 016Xg5  
Some improvements to the scheduling aspect of the model may be brought through !L@^Zgs|@?  
additional levels in the hierarchy for more detailed representation of the scheduling cLm{gd4 W  
activity. e@L?jBj8m  
It also unveiled new aspects of the role played by thyroid hormone in response to injury GtkZ%<KF9  
and tissue repair. ?Bsc;:KF  
In the near future, the ongoing clinical trials with siRNAs for macular degeneration and B^BbA-I  
RSV may reveal the exciting potential of RNAi therapeutics as the next major class of g/OL ^A  
drug molecules. ]t;bCD6*  
In the next few years it should become clear whether Q3aZB*$K  
It should be an exciting time for researchers seeking to harness this powerful endogenous ux1SQ8C*  
pathway to treat human disease. *+>QKR7  
Acknowledgement YHI@Cj  
The authors are grateful to the insightful work provided by Mr. John W. Harney and all FWuk@t[<O  
of the fellows and students that contributed with some of the studies discussed in this H6i;MQ  
article ,Cj` 0v#  
I would like to express my gratitude to all those who gave me the possibility to complete ?V(h@T  
this thesis. IGcYPL\&  
I want to thank the Department of Transport of the former Interim Government of [ h~#5x  
Namibia for giving me permission to commence this thesis in the first instance, to do the U$AV"F&!&}  
necessary research work and to use departmental data Oo8"s+G  
This work was supported by NIH grants AI058695 and AI056900. We thank members of aDVBi: _  
the laboratory and our collaborators for many useful suggestions. 4 X6_p(  
We thank J. Maraganore and B. Greene for critical reading of the manuscript, M. bs\7 juHt  
Duckman for graphics assistance and A. Capobianco for word processing assistance. >e9xM Gv  
Author contributions ky |Py  
All authors were involved in experimental planning and data analysis and contributed to 9 e'9$-z  
manuscript preparation. }pL#C  
Tables and Figures TaE&8;H#N  
Figure 7-1 sketches these relationships. yfCdK-9+B  
The graphical representation of these functions is shown in Figure 1. 6 xAR:  
The xx may be depicted as in Figure 1. !i*bb~  
Figure x shows the schematic diagram of the 8@- UvT&o  
Figure 1 though 2 provide a ... that fKjUEMRK  
the architecture of this expert system for .... is illustrated in Figure 2. WwYy[3U  
Figure 2 gives the outline of an ... system PcQqdU^!  
Table shows the Zh,{e/j  
27 @h";gN  
Time-dependent changes of '+*{u]\  
as shown in Table 1 and 2 :{ Q[kYj  
This concept is illustrated in Figure 2 @ob4y  
At the top of Table xx are shown two blocks of data. tNbZ{=I>  
Each table or matrix has constructs xx through xx as row;headings, szW85{<+  
xx through xx as column;headings. 8 s#2Zv  
Time course of calculated renin immunoreactive tissue volumes during development of A9L {c!|-  
the mouse kidney. Data are single values per time point. They were derived from the N% W298  
kidneys shown in Figure 1. "[t (u/e  
Yellow color indicates rS^+y{7  
PCT3 cells were pretreated with 50 M PD98059 or 10 M U0126 for 30 min and then ^B`*4  
cells were stimulated with 25 g ml-1 CsA for 6 h. ERK and PKB activation were MmPLJ  
analyzed by western blot using phospho-specific antibodies. F.[E;gOTo  
ERK and PKB activation were measured by western blot using phospho-specific wz{]CQ7"  
antibodies IGC:zZ~z  
The data are the means of three different experiments taking the number of untreated cells  Y3RaR 9  
(vehicle alone) as 100%. FT/STI  
ERK and PKB activation were then analyzed by western blot with the corresponding RC>79e/u<  
phospho-specific antibodies. To ensure equal amount of protein in each lane, western !9ytZR*  
blots against total protein were performed for ERK and PKB. SZ'2/#R>  
The means of optical density (OD) values of the bands detected in western blots of three 0V_dg |.  
different experiments are plotted, taking the maximal value of phosphorylation of ERK +J<igb!S  
and PKB as 100%. z;#]xC V  
CT3 cells were treated with 25 g ml-1 CsA or vehicle alone for 24 h and then fixed with Hk8pKpn3  
paraformaldehyde and stained with Hoescht as indicated in Materials and Methods. Then n?=d)[]  
cells were visualized by phase-contrast microscopy (upper panel) and fluorescence aUTXg60l*  
microscopy (lower panel). (o5^@aDr  
The data are the means of three experiments performed on different days taking the /3`(Ki{ Q  
number of cells at the onset of the experiment as 100%. 0 P]+/  
Rats were killed at 4 h and at days 1, 2, 4, 7, 9, 14, 21, and 28 after disease induction j{?ogFfi  
(n=9 each). Q eZg l!  
*P<0.05 versus non-nephritic rats. 9\J.AAk~/  
The expression of the CCN3 protein was detected by western blot analysis (n=4 each, PC, 9ER!K  
positive control, M, molecular weight markers). ={]tklND  
Original magnifications: 200 in A, E, G, H; 600 in B, C, D, F. lI*uF~ 'D  
Data are means s.d. of four independent experiments. WH6Bs=G\}  
*P<0.05 versus 0.5 h. 5H!6 #pqM  
PDGF-BB and -DD, but not PDGF-AA and -CC, induce a downregulation of CCN3 4?2$~\ x  
mRNA as determined by real-time RT-PCR and normalized to i+&o%nK2  
glyceraldehyde-3-phosphate dehydrogenase mRNA. &hco3HfW  
Cell number was counted in duplicate using a Malassez hemocytometer Ht_7:5v&   
Data are means s.d. of four independent experiments. *P<0.05 versus cells treated with &s>E~M0+J  
MCDB media for 24 h, #P<0.05. ucwUeRw,  
28 IL&;2%  
The curves are adjusted for history of coronary artery disease, CRP, and progression to hqwDlapTt  
ESRD. =`W#R  
Both models are independent of age, gender, baseline GFR and proteinuria, and other  I/`\>Hk  
clinical characteristics and traditional and nontraditional cardiovascular risk factors RJ0:O   
Correlation between serum bone-specific alkaline phosphatase (bAP) and serum PTH W6Z3UJ-  
(n=99, P<0.0001, r2=0.190) YU6D;  
Concentration of serum FGF23 concentration before starting and at the end of the 4-h f C_H0h3  
hemodialysis procedure in 23 patients, expressed as log10 values (n=23, P<0.0001, l`(pV ;{W  
Student's paired t-test). U%u%_{-  
Immunohistochemistry for the hypoxia marker pimonidazole (PIM) and HIF-2 ; %RD%AliO}K  
arrow=endothelial cell and CD=collecting duct <F6LC_  
Magnification: 1000. 3`U^sr:[%  
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CONJUGATION >4iVVs  
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additionally, again, also, and then, as can be easily understood, besides, equally important, \@F~4,VT  
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naturally, of course MNu\=p\Eq  
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still, traditionally, rather, unfortunately, yP0XA=,Y  
To Indicate Time Relationships u`Abko<D  
after a short time, afterwards, as indicated earlier, as long as, as soon as, at last, at length, 6S&OE k  
at the moment, at that time, at the same time, before, earlier, currently, immediately, in E@CK.-N|  
the meantime, in recent years, lately, later, meanwhile, often, of late, presently, recently, `L7Cf&W\l8  
soon, shortly, since, thereupon, temporarily, therefore, until, when, while SmCtwcB1  
29 ,u^%[ejH  
To Indicate Special Features or Examples g[ O6WZ!F_  
for example, for instance, incidentally, indeed, in fact, in other words, in particular, in Rg%Xy`gS  
practice, specifically, that is, to illustrate, in this respect, theoretically, as mentioned \W+Hzf] W#  
before / above 5 [X,?  
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in brief, in conclusion, in short, in summary, on the whole, to conclude KS Q*HO)5  
, in general, to summarize, to sum up, as a result, ultimately, e`bP=7`0  
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began a new era in ... =+w/t9I[  
can be regarded as / achieved / used to/for / found / obtained through |>Pz#DCy  
can result in l._g[qa  
carries out ... tasks FZtT2Z4 &i  
production information in order to simultaneously F%$l cQ04%  
contains all information necessary to describe j-zWckT{  
do not make use of production information SK 5__Ix  
deals with d(^3S>V|q  
end with failure jQ['f\R  
fetch the information from the model directly !eP0b~$/^J  
has great potential / yet to be resolved / spurred the development of / BirnCfj/2  
been recognized as x\pygzQ/  
site component / cable of / currently implemented for / demonstrated by an example / ?f*>=;7=  
finally reached / made equal to / equivalent to / more suitable to / oriented to / $]MOAj"LH  
interpreted as / pointed out / potentially of great benefit in the complex task of / shown Yu:($//w  
in / used to effectively guide the search xP &@|Ag  
makes use of -Fc 9mv(H  
was significantly higher as compared to syk,e4:oA  
To assess disease-specific alterations, we measured i}@5<&J  
To specifically assess the relationship between JC9OL.Ob  
make up OT#foP   
To determine if J7k=5Fqej;  
This statistically significant difference still :AyZe7:(D  
They positively correlated with \3whM6tK  
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30 P7!gUxcv9Y  
contribute to PRK*7-(  
meets the needs of real life production,/ the current demands placed upon it 4}Hf"L[ l  
must be justified ar@ysBy  
point out [Rzn>  
play an important role ?{>5IjL)en  
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will be available/ performed/ overlooked Y+"Gx;F>  
NOUN PHASE *1_A$14 l  
a basic technical function of  Fr9_!f  
a significant reduction of 6}PoBhgSg-  
drug variability sg,9{R ^  
a critical need +6 x:+9S  
negatively correlated with tNCKL. yU  
a key / principle feature of u1 (8a%ZC  
a substantial impact on vm"dE4W=  
an intensive review was conducted WK6|e [iP  
The analysis of the correlations by >"+bL6#  
an increasing need for expanding the application of .... NeY,O f|  
an important component / function / aspect / issue ;923^*\:F{  
each rule is numbered in sequence Hsz).u  
each of these involves z c, Q  
for this calculation, it is necessary to define Iw) 'Yyg  
in the physical environment / integration of -pm^k-%v  
in the reality of situations where … ]Q0+1'yuK  
many aspects of *>p#/'_E  
most past efforts have been spent on ... %1VfTr5  
common sense to a well studied and documented technical field. <',bqsg[  
sources of additional information on ... are listed Q@/358.LA  
systematic and rationally structured format xu7Q^F#u  
the basis on which a range of ...operations can be established is shown ,\n%e'  
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of / concept of ... has attracted wide interest / function is concerned with / heart / _ -/<bO  
impact / nature / role / task of / kernel functions ",qJG]_ <  
the number of parts needed to /xcl0oe(  
the above statement means that BTu_$5F  
the output data is passed to t!,GI&  
31 +Qe&#"O0  
the proposed method / underlying principle #$[}JiuL/  
the recommendations made in this report, if implemented, should m'i^BE  
this information resides in MKN],l N  
this process is composed of ... different ... operation To"J>:l  
along with the use of ZeyA bo  
concerning general aspects of 0'9z XJ"  
due to _ X* A  
for later use in generating..., Ev7fvz =  
in turn, *U=%W4?W  
it can be claimed/concluded that .\X;VWTI  
it demonstrates the decisions required of !w9w{dtW=  
it also provides information to .. 3M[b)At V.  
it becomes essential to _#8OHG.x  
let ... be the probability that VV1I2YcKt  
once... is written, it is compiled into... FX#fh 2  
suppose it is observed that _s_%}8o  
this is because QIMoe'p  
this results in a D^5bzZk N  
upon completion of the ... analysis, ]8qFxJ+2^  
when the knowledge is of mathematics or quantum physics, it will also be yLI)bn!"  
recorded in books and papers a\Ond#1p  
selection of rules for using the tools, for generating operation plans, ~ j`; $o  
is another matter of preference, since practice varies greatly. [Ie;Jd>gG  
for the sake of convenience K; +w'/{  
correct decision to be reach ,y+}0q-Ou  
keeping the number of rules to a minimum. : ;nvqbd  
a good process plan will result exhibiting several characteristics: /8(\AuDT  
practical solutions ' TF5CN X  
because of rather small job lot sizes Lt xeT .  
Backward reasoning can be used to answer the question "should milling tool be select" Y'YvVI  
different level of knowledge in the realm of process planning
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