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楼主  发表于: 2009-10-18   

医学SCI 论文经典句子

18 X{5vX T\/y  
Jurkat cells cultured in calcium-free RPMI-1640 medium (Gibco BRL; number Ag9 vU7  
22300-107) containing calcium-free 10% FBS were triggered by anti-Fas IgM. The l4F%VR4KT  
treated cells were harvested at the indicated time points and incubated with Fluo-3-AM at !%CWZZ 6u  
a final concentration of 1 micromolar for 30 min at 37°C (Scoltock et al., 2000). The s'!Cp=xQF"  
labeled cells (50,000 cells per treatment) were then analyzed by exciting the cells at 488 S0z D"T  
nm and examining the fluorescence emission of Fluo 3 at 530 nm with a FACS Scan, ZGexdc%  
(Becton Dickinson). A one micromolar concentration of LPA was used as a positive ?qPo=~y01  
control for Ca2+ induction. The data thus obtained was analyzed with the software Win MqW7cjg  
MDI 2.8 and represented as contour plots.The effect of chelating intracellular calcium on a zCf  
translocation of annexin I was studied by culturing Jurkat cells in the presence of 10 "|EM;o  
micromolar BAPTA-AM, with or without the addition of anti-Fas IgM. Cells were JZ)RGSG i  
harvested and fractionated as detailed above, and the S-100 fractions were assessed by |n2qVR,  
immunoblotting for presence or absence of annexin I. -.1y(k^4E  
Mouse bone marrow transduction and transplantation. Bal$+S  
Retrovirus-mediated transduction of mouse bone marrow cells was done by published J?V?R  
methods49. Prestimulated low-density bone marrow cells were infected with high-titer r(:5kC8K  
retrovirus supernatant on fibronectin-coated plates. Retrovirus supernatant was generated A 9( x  
in the phoenix-gp cells with a mouse stem cell virus–based retroviral vector coexpressing * aN  
EGFP and HA–RhoH as described50. EGFP+ sorted cells were transplanted by YN/|$sMD|  
intravenous injection into the sublethally irradiated (300 rads with a 137Cs irradiator) ?M8dP%&r  
Rag2-/- recipient mice. At 9 weeks after transplantation, thymus, peripheral blood, bone &TUWW/?T  
marrow, spleen and lymph nodes from each recipient mouse were collected for analysis p*< 0"0  
of EGFP+ chimerism and hematopoietic lineage by flow cytometry. Expression of ;y,5k?  
HA–RhoH and HA–RhoHF73F83 in EGFP+ sorted thymocytes of recipient mice was g2BHHL;`  
confirmed by immunoblot analysis. d(\%Os   
Determination of renal morphology {+ ][5<q  
Kidney slices were postfixed in buffered 2% OsO4, dehydrated, and embedded in an s]HOGJJz  
Araldite-EM bed 812 mixture. Large sections were cut perpendicular to the renal capsule, "i nd$Z`c  
containing cortex, and medulla. Thin (1 m) sections were analyzed in a blinded manner U_Vs.M.p  
for morphologic alterations, as previously detailed . k# U]M  
Patient population }u\])I3  
Patients included in the study met the following criteria: (1) biopsy-proven IMN; (2) m}C>ti`VD  
creatinine clearance 30 ml per min per 1.73 m2; and (3) persistent proteinuria >5 g per rA3$3GLQ-  
24 h despite treatment with an HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) tRs [ YK  
reductase inhibitor, an ACEi, and/or ARB at maximal tolerated dose for at least 4 months. _t iujP  
The Mayo Institutional Review Board and the Research Ethical Board, University Health rNrxaRQ  
Network, University of Toronto approved the study protocol. All patients gave written Nkjza:f{  
informed consent. Patients who had been on treatment with prednisone, cyclosporine, or S'k_olx7  
19 H['N  
mycophenolic mofetil within the last 4 months or alkylating agents within the last 6 VyXKZ%\dQ/  
months were not included in the study. Patients with active infection, diabetes, or a &:;:"{t}Do  
secondary cause of MN (for example, hepatitis B, systemic lupus erythematosus (SLE), U\ ig:  
medications, malignancies) were also excluded. )SaGH3~*C  
Treatment [glLre^  
At enrollment, a low-sodium (<4 g day-1) and low-protein (0.8 g per kg per day of rxe >}ZO  
high-quality protein) diet was recommended and patients were encouraged to maintain 9eGyyZg  
the same diet throughout the duration of the study. All patients received a similar 6w*dKInG[-  
conservative treatment regimen that included loop diuretics to control edema, an QCD .YFM  
HMG-CoA reductase inhibitor, and an ACEi combined with an ARB if tolerated. ? }Z1bH  
-Blockers and non-dihydropyridine calcium channel blockers, in that order, were added K`yRr`pW  
when required to control systolic blood pressures to <135 mm Hg in >75% of the 6o!!=}'E[  
readings. Patients who after a minimum of 4 months of conservative therapy and 3r<~Q7e  
maximized Ang II blockade had proteinuria >5 g per 24 h received two i.v. infusions of i/65v  
rituximab at a dose of 1000 mg on days 1 and 15. To minimize infusion reactions, o@r7 n>G  
patients were premedicated with acetaminophen (1000 mg) and diphenhydramine s28`OKC}  
hydrochloride (50 mg) orally. In addition, methylprednisolone (100 mg, i.v.) was given g$":D  
prior to the first rituximab infusion. B-cell depletion was defined as CD19+ count &Q%zl9g(g  
<5 cells per l at any time and B-cell recovery was defined as CD19+ cell count + @A  
>15 cells per l. Patients treated with rituximab, who at month 6 had proteinuria >3 g per ^T( .k=  
24 h and in whom CD19+ B-cell counts had increased to >15 cells per l, received a AhCW'.  
second course of rituximab treatment following the same protocol described above. 4qphA9i1  
Follow-up 3YeG$^y"  
In all patients, clinical and laboratory parameters including complete blood counts,  H_B4  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum gcii9vz `  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry g D0eFTN  
and at months 1, 3, 6, 9, and 12. Creatinine clearance and protein and creatinine excretion \6S7T$$ 1m  
in the urine were assessed by performing two consecutive 24-h urine collections at each P!~MZ+7#&  
time point. Data were considered accurate when urinary creatinine excretion was P]<4R:yb  
consistent with a complete 24 h collection. The mean of the two measurements was tf =6\p  
considered for the analysis. The presence of HACAs was evaluated at baseline and at y>R=`A1b  
months 3, 6, 9, and 12. hv)x=e<  
Method / Approach / Study/ Technique Y_Eb'*PY  
A discussion is presented of a problem-solving system Hj'xAtx5  
To improve the efficiency of the method, the following approach may be applied. \K_ET> !  
In order to an investigation was made to find the causes of the AG3>V+k{Lv  
Although large collections of rules and equations have been complied, none are generally Gn22<C/  
accepted "HX<,l8f%  
20 ];}Wfl  
This approach will be explained and discussed thoroughly in the body of the report. A]y`7jJ  
This can be accomplished by &d9{k5/+\  
This algorithm to compute the total cost can be described step by step as follows: osciZ'~  
The above preliminary analysis has provided important information yxonRV$&  
Various methods have been proposed for selecting an optimum... sI 43@[  
These concepts have been applied to Q.bXM?V)  
On the basis of the concept mentioned above, pEw"8U  
This can be achieved by < 3(LWxw  
In addition, tissues were stained for infiltrating lymphocytes (CD20 and CD3), and the []x#iOnC&  
amount of interstitial fibrosis was quantified by histomorphometry. Formalin-fixed, XoXM ^*Vk  
paraffin-embedded sections were cut onto coated glass slides. Following heat-induced -_ I _W&  
antigen retrieval, sections were incubated at 20°C overnight with either anti-CD20 KTK <gV9:  
primary antibody or anti-CD3 primary antibody, both at 1:1000 dilution (Dako, Canada %/EVUN9=  
Inc, Mississauga, Ontario, Canada). After rinsing all sections, pretreatment with 3% |HU@ >  
hydrogen peroxide was performed to prevent endogenous peroxidase activation. Sections H!vax)%-\  
were incubated with a secondary rabbit anti-mouse antibody linked with avidin–biotin liEPCWl&  
complex. Sections were counterstained with hematoxylin and examined by light d[r#-h> dS  
microscopy. D@C-5rmq  
The HACA assay is a proprietary bridging enzyme-linked immunosorbent assay fz;iOjr>  
performed at Genentech Inc. that measures the antibody response to rituximab in human YgKZ#?*  
serum samples. mrTlXXz  
In all patients, clinical and laboratory parameters including complete blood counts, <Q$@r?Mu]  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum $GYm6x\4  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry !P ~_Dl2d  
and at months 1, 3, 6, 9, and 12. g$n7CXoT  
This fact suggests that a new concept 2<li7c59  
This was accomplished by taking ... aF8fqu\  
The preparatory stage is very time consuming process. Yj%U >),8  
Test are performed for validity, completeness, and compatibility 'AzDP;6qFI  
There is little hope of achieving successful ... KN$}tCU  
There has been an increasing awareness of the potential of using most ..so far made have r/& sub"X  
not taken this approach, with the exception of n 9\ C2r  
Only a few studies can be found. j0Bu-s O$w  
It is a very tedious process to go through og4UhP^UET  
It is only when .. has been completed that .. may be effected dG@"!!,  
The entire interpretation process is conducted in one's head. ()t p>  
These approaches are sometimes very tedious. DQMHOd7g  
Several techniques can be used ?/TSi0R  
A polynomial parametric model can be written as [the following]/[follows]: 0$_oT;{8  
A xx model is constructed/formulated using xx. *ohL&'y  
A xx model represents an xx by its xx. OQ8 bI=?[x  
A process decision model captures the logic essential to -y R.<KnL  
From the equation above, xx is equal to the summation of xx times the ... $\b$}wy*  
21 t(GR)&>.2  
The validity of a xx model can be checked using Euler's formula. x??pBhJH  
Given a model, one can mathematically determine whether ... or ...  ~UyV<  
Equations for xx need to be derived and implemented in the system. <Gav5R c  
A number of heuristic rules have been developed for MWI4Y@1bS  
Optimum .. techniques can be made more reliable by ... so that sBu=e7  
An algorithm based on the characteristic ... is used to determine >a"Z\\dF  
Euler's formula states the following: "+\lws  
The completed model should agree with the formula. qud\K+  
For manufacturing purposes, a detailed and precise model of the object is necessary o]Ol8I  
Engineering design models are very well defined; therefore, MepuIh  
To keep the domain narrow enough to be implementable, yet wide enough to be useful. {*[\'!d--.  
Point of View m.px>v-  
from an implementation standpoint, p`nPhk,:b  
From the point of view of this application, Vm5c+;  
From this point of view, Zadeh suggested an inference rule named xxx (CRI for short). V?Z. \~  
Information is the meaningful interpretation and correlation of some aggregation of data qP2ekI:y  
in order to allow one to make decisions. )foq),2  
From a practical point of view, the computational aspects of an FLC require a i O/K nH  
simplification of the fuzzy control algorithm. {n/uh0>f*  
The use of a hammer to insert screws, although partly effective, tends to distort, destroy, I/M_p^  
and generally defeat the purpose of using a screw [Kusiak AI Implications for CIM jx.[#6e  
p.129] O:TlIJwW  
Statistical analysis CqHK%M  
Data were analyzed by one-way analysis of variance comparing the three conditions ~zil/P8  
(sham operation, ischemic AKI, and bilateral nephrectomy) at each time point. If %YSpCI  
significant F-statistic from analysis of variance existed, this test was followed by Dunnett %uJ<M-@r=u  
post hoc multiple comparison procedure with sham operation as the control group. For all \%/#x V  
other comparisons, Student's t-test was used. A P-value of <0.05 was considered as 2H/Z_+\  
statistically significant. BBH0OiV=  
Values are expressed as means s.e.m. and significance was evaluated by Mann–Whitney !>BZ6gn5  
U test using GraphPad Prism, version 4.0 software (GraphPad Software Inc., San Diego, =8r 0 (c  
CA, USA). S!Omy:=;i  
All values are expressed as means s.d. Statistical significance (defined as P<0.05) was Vw#_68EybM  
evaluated using analysis of variance and Bonferroni t-tests, and the two-tailed Pearson's CM<]ZG7  
test, where appropriate. YLwnhy>dD  
Data are expressed as mean s.d., median and interquartile range, or frequencies, as |+35y_i6  
appropriate. Variables who deviated from the normal distribution (positively skewed) +Vo}F  
were log-transformed (log10) before the correlation study. OkCQ?]  
Data are represented as means s.e.m. Student's t-test and multiple comparisons with t-test ndjx|s)E  
post hoc analysis of variance were used as indicated below, for the comparison of 'fcMuBc+ 4  
22 FP0G]=ME  
morphological, immunohistological and functional parameters. Statistical significance {fha`i  
was set at P<0.05. R)M_|ca  
The primary efficacy parameter was defined as change in urinary protein excretion from Xcrk;!IB?  
baseline (week 0) to 12 months after treatment. The 12-month changes were tested f;&]:2.j  
against zero using the paired t-test. Secondary end points included 6-month changes in -6tgsfEr  
protein; the number with PR or CR at 6 or 12 months; and changes in glomerular "V>R9dO{"!  
filtration rate (GFR), serum albumin, and lipid profiles. Study sample size was based on cTXri8K_  
the desire to have 80% power to detect a drop in urinary protein of at least 2.0 g day-1. H;U)b{  
Assuming a two-sided hypothesis test performed at a significance level of 0.05 and an s.d. o\88t){/kB  
of urinary protein change of 2.5 g, it was determined that 15 patients were required.2 L lw&& K  
Definition of remission status is according to the criteria established by Cattran et al.30 ,h{A^[yl  
CR was defined as proteinuria <0.3 g per 24 h, PR as proteinuria 3 g per 24 h, and a kloR#?8A  
>50% reduction in peak proteinuria and non-response as <50% reduction in peak  Vs)--t  
proteinuria. Any patient reaching a CR or PR was considered a treatment success !eAo  
The statistical significance of differences for the mean values of cytokine concentration r6;$1 K*0  
and T cell proliferation was determined with Student's t-test. Differences with a P value '8 fk+>M  
of less than 0.05 were considered significant. 7}GK%H-u  
Statistical significance was determined by Student's t-test. Data with a P value of less 6ns! ~g@  
than 0.05 were considered significant. "F3]X)}  
In vitro and in vivo experiments were analyzed by the two-tailed Student's t-test, with P mCs#.%dU  
values less than 0.05 considered statistically significant. {TOmv  
The significance of differences among groups was determined by Student's t-test and SFb{o <0 =  
one-way analysis of variance (SigmaStat software; Jandel). Cv>o.Bp|  
Comparisons .fi/I  
As well, the pros and cons of these representations from a process planning point of view B aO1/zk  
will be discussed. [L6w1b,  
The method of using xx to implement xx described by Zadeh (1973) appeared more /Y&02L%\3s  
suitable  cO\-  
As discussed [in the previous section]/[preciously], FVB;\'/  
Relation 0l=+$& D  
We can not invert F' directly because it defines a many-to-one mapping. ?!=iu!J  
The relationships appear very complicate x lsqj`=  
Lifting tasks involve complex and imprecise relationship between the task variables and R#n%cXc|  
the human operator's characteristics. R8-^RvG  
These methods are based on the relationship between ... and ... !)qQbk  
The fundamental concept of a fuzzy rating language is that we can establish a relationship aup6?'G;  
among terms such as high, medium, and low, and then modify these relationships. DY{cQb  
This article will thus mention the latter as well as the former. KtB!"yy#  
The former two bear a close relation to a fuzzy Cartesian product. )9B:wc"  
Importance HPQ/~0$  
G ){g  
23 Fl{WAg  
The emphasis is on an implementation of a general approach to rule based decision B/J&l  
making. IN7<@OS7  
Consideration / Attention 9p@C4oen  
Careful evaluation is necessary to ensure V8-h%|$p3W  
Such a formulation does not change further considerations. BHj\G7,S  
Considerable attention has been paid to z *9FlV  
Attention should be paid to an important finding of this investigation. /%n`V  
Caution should be exercised in this process to avoid ... 1%J.WH6eQ  
Primary consideration is given to ... components, though others can be accommodated ~vgA7E/XV  
After ... has been defined by ..., a carefully analysis is carried out/performed to determine I,ci >/+b  
A number of factors such as ...need to be taken into consideration before making the k$Rnj`*^  
appropriate decision. ]lS@}W\  
It should be noted that k_GP> b\"k  
It is important to point out that ... 8I+d)(:  
These considerations have heightened interest in the possibility of providing ... ?Qqd "=k4  
We should stress the fundamental importance of the xx 'GJVWpvUU  
Results. ~2uh'e3  
Advantages / Disadvantage Uh|>Skic4  
One of the major advantages of this new measure of xx is that it can be applied to the BRbV7&  
experimental study of XidxNPz 0^  
One advantage of using a .. is the ease of preparing it. F9XT lA  
It has a very fast decision making process Vf-5&S&9  
All the algorithms involve mostly logical operations. cs_}&!c{  
It can be easily and without additional cost implemented in a microprocessor;based y/_wx(2  
environment. oP,9#FC|(  
It can reduce the waste of designing from scratch. `_ (~ Ud  
The advantages of using a xx to represent xx are the following: VY'Q|[  
However, xx is not without its shortcomings. x:5dC I  
In most cases, the xxx shows an improvement over the existing xxx. tSv0" L  
Compared to the existing xx, the impacts of the xx are generally reduced by 5% to 9%. _$/ +D:K  
The "best case" results shows a savings of 6% to 9%. X QbNH~  
Most of the existing works based on xx approach can only recognize a xx . _zC (J  
Most of the above methods are computational expansive and limited to xx. j%&  IL0  
Some other advantages of xx are the following: &K:' #[3V  
The problem is the limitation of this method to a limited domain of parts. m/USC'U%  
It proved limited in application because it demanded precision in system modeling that *,#q'!Hq  
was impossible in practice. 0^_MN~s(X  
There are advantages to be gained in the structuring of costs and benefits, the use of xx, PDw{R]V+  
The disadvantages of this method are also disadvantages of conventional xx approaches. ]1fZupM^6  
This combines the best features of both techniques 9Z!lmfnJ  
24 g0#q"v55  
Hopefully, this tool can be as the reference framework of for developing a xx platform, z] @W[MHY  
and helping the administration, marketing, and knowledge management activities in rm+v(&  
virtual communities. EI`vVI  
Results Sa]Ek*  
An improvement on the result shown above can be made by based on the data provided {HZS:AV0  
Recent work has identified eihZp  
Time-dependent changes of ySruAkw%  
Cyclosporine-induced cell death is triggered by a non-classical phosphoinositide 3-kinase %{ ~>n"  
and does not require ERK activation. k\(4sY M  
Much of the current work on  tSw>@FM  
Discussion of these theories is beyond the scope of this review rycJyiw<-  
Based on the information contained in this G:Hj;&'2  
The result can be categorized into nine classes DJ:38_F  
The results are illustrated by an example _O 52ai><b  
The experimental results for each xx time are reported in Table 2. ZDlu1>Q  
From the results obtained so far, it seem that SL*DK.  
Because of the inaccuracy of the ..., a conclusion cannot be drawn as /Nq rvy=  
Although much effort has been made to., this reality is far from completion. lS{4dvr?w  
The results indicate that the total benefits are higher than the total costs. -c$z 2Q)  
Their results may then serve as guidelines for lower level models, less fuzzy and more S1C^+Sla]  
detailed. 3LfTGO  
Conclusion O|(o8 VS  
From the discussion, one may conclude that ... Gmgeve  
The first indication that XZBj=2~-3  
In summary, we have shown that AFtCqq #[  
These results suggested that B}k'@;G  
Our findings have shown that `wV|q~  
Our observations have provided Cji#?!Ra?  
This study reveals following five main findings: o~}1 oN  
To our knowledge, this is the first immunohistochemical report of both PIM and HIFs in ?k w/S4  
the diabetic kidney. YZ{jP?x  
As mentioned above, T"A^[ r*  
Moreover, we demonstrate that, <!hpfTz*  
A number of studies have elaborated a body of knowledge about y $v@wb5  
There are findings to indicate that XL?A w  
On a descriptive level, there is agreement that for E.4n}s  
One issue that became particularly evident from our study is k{qLkcOg=  
Our data are in agreement with the findings and models presented by previous @!-aR u  
publications, in particular those of Jones et al UJ9q-r  
According to our observations, wC(XRqlE  
…was not evident from our data sMN>wbHwh[  
25 t3Qm-J}wSB  
It could therefore be concluded that B[Ix?V4yy  
our data illustrate that sOjF?bCdO  
All these different aspects, as listed above, lead to 1wU=WE(kKZ  
In fact, it has already been speculated that 3[iSF5%V*p  
It should be furthermore mentioned in this context that `6n!$Cxo  
To identify and verify the essential factors triggering developmental renin expression, it PGMv(}%;  
is of interest now to study the development of intrarenal renin expression in mice with T&mbXMN  
defined gene mutations, using the results of this study as a reference system. T^+1rG  
In conclusion, using several approaches, we demonstrated that h<Jc;ht  
But we have not provided formal proof yet that =]WW'~  
In summary, our results suggest that Q R|XV%$  
In conclusion, we identify 2z[r@}3  
A better understanding of such regulatory systems may yield novel therapeutic D8q3TyCj%  
approaches to glomerular diseases. ;K>{_k f  
Preliminary results of short-term studies (2 weeks) indicate that kT>r<`rt  
However, we believe that 9$:QLE+t  
However, despite our extensive measurements of these parameters, we found no B-B?Ff>  
relationship between response and initial proteinuria, time to B-cell recovery, the :QxL 9&"  
development of antibodies to rituximab, B-cell count in the kidney tissue, nor the degree :Pj W:]  
of tubular interstitial damage present. A clear conclusion for efficacy, however, cannot be u'#`yTB6b  
obtained from an uncontrolled study, and definitive claims of efficacy should be reserved %H 8A=  
for rigorous, prospective, controlled, and randomized trials with the use of rituximab and DN4fP-m-  
that will also look for factors that may determine its efficacy in IMN. cZCGnzy  
It has become increasingly clear that *4r s  
Form the above discussion, the conclusion can be reached that 03P N{<  
The conclusions drawn are also valid }R7sj  
In conclusion to this, it becomes obvious that the problem of xx lies not only in... }<6oFUZ  
We have attempted to introduce some concepts associated with a theory of 7KvXTrN!9  
Considerable more work, hopefully, will be done in this area -d$8WSI 8  
Employing the compositional rule of inference, the assessment of the xx compatibility in >cGh|_9  
achieving prescribed xx projectiles in any level of the hierarchy is made possible. Z?!JV_K  
This paper has presented a theoretical and experimental study of the xx process and xx U:s} /to  
concept. <^H1)=tlF  
The experimental research results will hopefully serve as useful feedback information for \O 8Y3|<  
improvements for xx work. {^PO3I  
The scope of this contribution was to introduce a xx method. Z XF AuF  
This has a tremendous impact on our understanding of gaLEhf^  
Significant progress has been made in advancing RNAi therapeutics in a remarkably XgwMppacw  
short period of time o 2[vM$]  
To date, little is known about… hLDch5J5~  
Irrespective of the mechanisms and the molecules –which are still largely unknown – CT$& zEIm  
involved in MSC functions, current data suggest that 1YF+(fk  
To better understand hQd@bN8  
26 3{2^G@j  
Future Research (常用于conclustion 的结尾) h"RP>fZt  
Thus, first extension of the approach could be, E<X{72fb>  
Further studies are needed to determine the importance of = )JVT$]w  
Some improvements to the scheduling aspect of the model may be brought through Rye ~w6  
additional levels in the hierarchy for more detailed representation of the scheduling I =tyQ`  
activity. [*Aqy76Qa  
It also unveiled new aspects of the role played by thyroid hormone in response to injury m>Yo 9/XpZ  
and tissue repair. 1_33;gP  
In the near future, the ongoing clinical trials with siRNAs for macular degeneration and i-:8TfI,  
RSV may reveal the exciting potential of RNAi therapeutics as the next major class of Tc'{i#%9j  
drug molecules. RpmBP[  
In the next few years it should become clear whether  Gh)sw72  
It should be an exciting time for researchers seeking to harness this powerful endogenous .b_0k<M!p  
pathway to treat human disease. Q+u#?['  
Acknowledgement P/C+L[X=  
The authors are grateful to the insightful work provided by Mr. John W. Harney and all tn:/pPap  
of the fellows and students that contributed with some of the studies discussed in this :XPC0^4s  
article r {/ G\  
I would like to express my gratitude to all those who gave me the possibility to complete _v~D {H&}  
this thesis. 7T|J[W O  
I want to thank the Department of Transport of the former Interim Government of /IrR,bvA  
Namibia for giving me permission to commence this thesis in the first instance, to do the M3jv aI  
necessary research work and to use departmental data HP4'8#3o  
This work was supported by NIH grants AI058695 and AI056900. We thank members of 3x(MvW30Lg  
the laboratory and our collaborators for many useful suggestions. r:WgjjA%  
We thank J. Maraganore and B. Greene for critical reading of the manuscript, M. -hU>1ux&V  
Duckman for graphics assistance and A. Capobianco for word processing assistance. ?sjZ13 SUa  
Author contributions cAKoPU>U  
All authors were involved in experimental planning and data analysis and contributed to w.T=Lzp  
manuscript preparation. Kl46CZs#8  
Tables and Figures z|DA _dG  
Figure 7-1 sketches these relationships. +/~\b/  
The graphical representation of these functions is shown in Figure 1. z[|PsC3i:  
The xx may be depicted as in Figure 1. $cJN9|$6  
Figure x shows the schematic diagram of the $)TF,-#x  
Figure 1 though 2 provide a ... that OnPy8mC  
the architecture of this expert system for .... is illustrated in Figure 2. e?? {&[  
Figure 2 gives the outline of an ... system f5=t*9_-[  
Table shows the c~L6fvS  
27 s68_o[[E  
Time-dependent changes of $?/Xk%d+  
as shown in Table 1 and 2 uuUVE/^V'  
This concept is illustrated in Figure 2 d*\C^:Z  
At the top of Table xx are shown two blocks of data. uVuToMCp  
Each table or matrix has constructs xx through xx as row;headings, ap'kxOf"1  
xx through xx as column;headings. 0Yzb=QMD  
Time course of calculated renin immunoreactive tissue volumes during development of "! 6 B5Oz  
the mouse kidney. Data are single values per time point. They were derived from the ,^+R%7mv  
kidneys shown in Figure 1. ad$Qs3)6o  
Yellow color indicates *liPJ29C[  
PCT3 cells were pretreated with 50 M PD98059 or 10 M U0126 for 30 min and then :5cu,&<Gv  
cells were stimulated with 25 g ml-1 CsA for 6 h. ERK and PKB activation were +&N&D"9A  
analyzed by western blot using phospho-specific antibodies. @aD~YtL"n  
ERK and PKB activation were measured by western blot using phospho-specific \]`(xxt1  
antibodies +|"n4iZ!)  
The data are the means of three different experiments taking the number of untreated cells B]KLn?zt5  
(vehicle alone) as 100%. h%w\O Z7  
ERK and PKB activation were then analyzed by western blot with the corresponding 3JE;:2O~P  
phospho-specific antibodies. To ensure equal amount of protein in each lane, western hv:Z%D |S  
blots against total protein were performed for ERK and PKB. bq6{ty"  
The means of optical density (OD) values of the bands detected in western blots of three 4tTZ kJc  
different experiments are plotted, taking the maximal value of phosphorylation of ERK 33KPo0g7  
and PKB as 100%. rDx],O _  
CT3 cells were treated with 25 g ml-1 CsA or vehicle alone for 24 h and then fixed with '5,,XhP  
paraformaldehyde and stained with Hoescht as indicated in Materials and Methods. Then j_WF38o  
cells were visualized by phase-contrast microscopy (upper panel) and fluorescence /qq&'}TZP  
microscopy (lower panel). *T2&$W|_a  
The data are the means of three experiments performed on different days taking the ^57fHlw  
number of cells at the onset of the experiment as 100%. --%2=.X=  
Rats were killed at 4 h and at days 1, 2, 4, 7, 9, 14, 21, and 28 after disease induction WZ6{(`;#m  
(n=9 each). o>A%}YU  
*P<0.05 versus non-nephritic rats. KZ}4<{3  
The expression of the CCN3 protein was detected by western blot analysis (n=4 each, PC, [;#.DH]  
positive control, M, molecular weight markers). g+/U^JIc4l  
Original magnifications: 200 in A, E, G, H; 600 in B, C, D, F. =i5:*J  
Data are means s.d. of four independent experiments. (l P4D:X  
*P<0.05 versus 0.5 h. KP7RrgOan&  
PDGF-BB and -DD, but not PDGF-AA and -CC, induce a downregulation of CCN3 PRlo"kN  
mRNA as determined by real-time RT-PCR and normalized to IC-xCzR  
glyceraldehyde-3-phosphate dehydrogenase mRNA. wG?kcfu  
Cell number was counted in duplicate using a Malassez hemocytometer @?=)}2=|?i  
Data are means s.d. of four independent experiments. *P<0.05 versus cells treated with U1|4vd9  
MCDB media for 24 h, #P<0.05. '*ICGKoT  
28 tWOze, N  
The curves are adjusted for history of coronary artery disease, CRP, and progression to i8 ):0  
ESRD. >(ww6vk2  
Both models are independent of age, gender, baseline GFR and proteinuria, and other 2y7q x1$C  
clinical characteristics and traditional and nontraditional cardiovascular risk factors V1>94/waa  
Correlation between serum bone-specific alkaline phosphatase (bAP) and serum PTH 2,Dc]oj  
(n=99, P<0.0001, r2=0.190) EF=D}"E6pO  
Concentration of serum FGF23 concentration before starting and at the end of the 4-h bNU^tL3QZ  
hemodialysis procedure in 23 patients, expressed as log10 values (n=23, P<0.0001, ~+nSI-L  
Student's paired t-test). v 4b`19}  
Immunohistochemistry for the hypoxia marker pimonidazole (PIM) and HIF-2 ; [=1?CD  
arrow=endothelial cell and CD=collecting duct RS02>$jo  
Magnification: 1000. oNsx Fi:  
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CONJUGATION vm^# aoDB  
To Indicate Addition ,:4w$!;  
additionally, again, also, and then, as can be easily understood, besides, equally important, E d/O\v@  
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likewise, moreover, next, second, third, too, evidently, obviously, roughly speaking, :{uUc  
broadly speaking @N{Ht)1r  
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accordingly, as a result, consequently, for this reason, hence, in short, otherwise, then, kj=2+)!E7  
therefore, thus, truly TC#B^m`'p  
To indicate Comparison B "zg85 e  
in a like manner, likewise, similarly , alternatively #7T={mh  
To Indicate Concession eSBf;lr=  
after all, although this may be true, at the same time, even though, even so , I admit, X(z-?6N4  
naturally, of course 6yM dl~.  
To Indicate Contrast ,ToEK Id  
and yet, at the same time, but, for all that, however, yet, in fact, in contrast, in the real life, U7eQ-r  
in spite of, nevertheless, notwithstanding, normally, on the contrary, on the other hand, kP@OIhRe  
still, traditionally, rather, unfortunately, W3rvKqdw5  
To Indicate Time Relationships ;<<IXXKU  
after a short time, afterwards, as indicated earlier, as long as, as soon as, at last, at length, 2`m_"y  
at the moment, at that time, at the same time, before, earlier, currently, immediately, in 6&0a?Xu  
the meantime, in recent years, lately, later, meanwhile, often, of late, presently, recently, I|;#VejX  
soon, shortly, since, thereupon, temporarily, therefore, until, when, while Y,\mrW}K   
29 (Fd4Gw<sq  
To Indicate Special Features or Examples l'\b(3JF  
for example, for instance, incidentally, indeed, in fact, in other words, in particular, in a8aqcDs>O  
practice, specifically, that is, to illustrate, in this respect, theoretically, as mentioned )ixE  
before / above i=QhX CM  
To Indicate Summary ttVSgKAsm  
in brief, in conclusion, in short, in summary, on the whole, to conclude QLG,r^  
, in general, to summarize, to sum up, as a result, ultimately, =oDrN7`,B  
VERB PHASE  B4ze$#  
build a .. model { rn~D5R  
have been described to iL1so+di  
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we detected fZgU@!z  
build up the key link 9 `&D  
Although a number of studies have identified predictors of ^%JWc 3jZ  
began a new era in ... 6^Vf 5W{  
can be regarded as / achieved / used to/for / found / obtained through g XMkI$ab  
can result in mJ7kOQ-.$  
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production information in order to simultaneously a!]%@A6p  
contains all information necessary to describe 0 fU>L^P_?  
do not make use of production information a@J :*W  
deals with u*`GIRfWT  
end with failure -<=< T@,  
fetch the information from the model directly 5{TF6  
has great potential / yet to be resolved / spurred the development of / (tN$G:+")F  
been recognized as m=V2xoMw6  
site component / cable of / currently implemented for / demonstrated by an example / K%Bi8d  
finally reached / made equal to / equivalent to / more suitable to / oriented to / {o`5&EoM  
interpreted as / pointed out / potentially of great benefit in the complex task of / shown a\E]ueVD2j  
in / used to effectively guide the search H#E0S>Jw|  
makes use of  P_g  
was significantly higher as compared to * =l9gv&  
To assess disease-specific alterations, we measured pp jrm  
To specifically assess the relationship between [bXZPIz;j  
make up Fu$sfq  
To determine if jtwO\6 t&  
This statistically significant difference still i^l;Pv IF  
They positively correlated with Op{Mc$5a  
Present ."O(Ig[  
Our results are consistent with our recent data that suggest that <0m;|Ai'W  
30 Kw|`y %~  
contribute to { L5m`-x  
meets the needs of real life production,/ the current demands placed upon it &#l M$7/  
must be justified /2@@v|QL  
point out w[&BY  
play an important role >smaR^m  
relates to 79DC]48M  
rely on y (A"g3^=  
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satisfy the needs BJ5#!I%h  
determine the total requirements for the ... mN`a]L'  
uses ... as a key to search for... T$e_ao|  
without relying on P$bo8*  
will be available/ performed/ overlooked 5tL6R3  
NOUN PHASE iu'yB  
a basic technical function of (hoqLL\}k  
a significant reduction of >/*\x g&J  
drug variability __M}50^  
a critical need [g? NU]  
negatively correlated with Xqy{=:0  
a key / principle feature of ` T!O )5  
a substantial impact on |*b8-a8<  
an intensive review was conducted `6/7},"9t  
The analysis of the correlations by &V"&SV>}  
an increasing need for expanding the application of .... ]:;gk&P  
an important component / function / aspect / issue gS%J`X$  
each rule is numbered in sequence ;3!TOY"j;e  
each of these involves seq$]  
for this calculation, it is necessary to define a'Z"Yz^Eo  
in the physical environment / integration of F2&KTK  
in the reality of situations where … L`\ILJz  
many aspects of iAN#TCwLT7  
most past efforts have been spent on ... ,oC r6 ]  
common sense to a well studied and documented technical field. Ah,Zm4:  
sources of additional information on ... are listed yVL~SH|  
systematic and rationally structured format `{ /tx!  
the basis on which a range of ...operations can be established is shown e\89;)  
THE basic philosophy / principles of / key element / general hypothesis / candidate list +#W5Qb}VR  
of / concept of ... has attracted wide interest / function is concerned with / heart / -+L1Hid.7  
impact / nature / role / task of / kernel functions by {~gu  
the number of parts needed to */1z=  
the above statement means that jkbz8.K  
the output data is passed to +RbCa c  
31 dCHU* 7DS  
the proposed method / underlying principle u^ T2  
the recommendations made in this report, if implemented, should 0<Y)yNsV  
this information resides in d; M&X!Y  
this process is composed of ... different ... operation k: {$M yK  
along with the use of 6ul34\;  
concerning general aspects of  6} 9A0  
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for later use in generating..., y]/{W}D  
in turn, Q/< $ (Y  
it can be claimed/concluded that 3}H94H)]a  
it demonstrates the decisions required of 0q/g:"|j  
it also provides information to .. P6X 4m(t  
it becomes essential to 4'{j'kuv  
let ... be the probability that `0sa94H1[  
once... is written, it is compiled into... X1+Wb9P  
suppose it is observed that Tj>~#~  
this is because >%7iL#3%  
this results in a !D1F4v[c=  
upon completion of the ... analysis, I3SLR  
when the knowledge is of mathematics or quantum physics, it will also be ZJ!/49c*>  
recorded in books and papers  Jl}$) '  
selection of rules for using the tools, for generating operation plans, 8(BLS{-"<  
is another matter of preference, since practice varies greatly. Jk`A}  
for the sake of convenience N ^)L@6  
correct decision to be reach ><$hFrR!  
keeping the number of rules to a minimum. #j@Su )+  
a good process plan will result exhibiting several characteristics: qwnC{  
practical solutions C[~b6 UP  
because of rather small job lot sizes |vzGFfRI  
Backward reasoning can be used to answer the question "should milling tool be select" Yh4e\]ql~N  
different level of knowledge in the realm of process planning
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